Haimeng study Industrial Co., Ltd. Supply of trypsin

Description Haimeng study Industrial Co., Ltd. of quality manufacturers, phone manufacturers trypsin, trypsin CAS number, trypsin, trypsin Shanghai latest offer, the price of trypsin, trypsin, trypsin factory distributor, trypsin manufacturers latest offer, the molecular weight of the amount of trypsin, trypsin suppliers, trypsin. English: Trypsin vitality: 10000U / g CAS: 9002-07-7. Introduction Chinese name: Trypsin Chinese Synonyms: trypsin; Trypsin 1: 250 (pig pancreas); trypsin 1: 300 (pig pancreas); pancreatic amylase; pancreatic juice enzymes; -EDTA trypsin solution; trypsin 1 : 250; proteases CAS: 9002-07-7 Formula: C6H15O12P3 Molecular Weight: ≈24000 Introduction: as a protease, EC3.4.21.4. In the crest vertebral animals, and acts as a digestive enzyme. It is used as an enzyme in the pancreas trypsinogen precursor was synthesized. As a component of pancreatic juice secreted by enterokinase or trypsin decomposed to limit the activation of trypsin, the peptide chain endonuclease, it can polypeptide chain carboxyl side of lysine and arginine residues cutting. It not only plays the role of digestive enzymes, but also limit the decomposition of precursor chymotrypsinogen, procarboxypeptidase, plasminogen and other enzymes of phospholipids, from the activation. Is the most specific protease, a protein amino acids in the decision arrangement, it becomes an indispensable tool. Introduction of bovine trypsin amino acid residues 223, molecular weight 23,300, the active site serine residue is indispensable serine proteases. In addition to crest exist in vertebrates, but also present in the silkworm, starfish, 蝲 Regardless, actinomycetes wide range of organisms. Also with higher animal blood coagulation and inflammation associated thrombin, plasmin, vasodilatory factors such as protease specific chemical structure and so has a close relationship with trypsin, can think that these enzymes are enzymes from a common ancestor In the evolutionary process of differentiation comes. Chymotrypsin and elastase in terms of structure and catalytic mechanisms are closely related, but the specificity is completely different. Trypsin department of a proteolytic enzyme extracted from cattle, sheep or pig pancreas. Chinese pharmaceutical standards Dry goods, each 1mg potency is not less than 2500 units. Extracted from cattle, sheep, swine pancreas derived from a peptide chain endonuclease, broken only lysine or arginine carboxyl involved in peptide bond formation. White or beige crystalline powder. Dissolved in water, soluble in ethanol, glycerin, chloroform and ether. Molecular weight of 24 000, pI 10.5, the optimum pH of about 7.8 to 8.5. pH> 9.0 irreversibly inactivated. Ca2 + has a stabilizing effect on the enzyme activity; heavy metal ions, organic phosphorus compounds, DFP, natural trypsin inhibitor strongly inhibits its activity. Clinically used anti-inflammatory swelling, for leather, silk processing, food processing industry. The role and use product proteolytic enzyme that selectively hydrolyzed proteins by peptide lysine or arginine carboxyl constituted, can digest dissolve denatured egg quality of undenatured protein had no effect, therefore, can make pus, sputum, blood clots and other decomposition, thinning, easy drainage excluded, accelerate wound purification, promoting granulation tissue regeneration, in addition to anti-inflammatory effect. For local edema, hematoma and abscess, empyema, hemothorax, surgical inflammation, ulcers, traumatic injuries, such as arising fistula clinic. Inhalation spray, for respiratory diseases. It can also be used to treat snake bites. Also commonly used in animal cell culture for tissue before treatment. Cell culture: the role of trypsin is to proteolysis between cells so that the cells were discrete. Different tissues or cells in pancreatic effect on the reaction is different. Trypsin dispersed cell activity also its concentration, temperature and time dependent, at pH 8.0, temperature 37 ℃, the role of trypsin solution is strongest. When using trypsin, should take a good concentration, temperature and time, in order to avoid digestion caused by excessive cell damage. Because Ca2 +, Mg2 +, and serum protein can reduce the activity of trypsin, trypsin solution should be chosen so that the preparation of free Ca2 +, Mg2 + in the BSS, such as: D-Hanks solution. Upon termination digestion, available serum-containing medium or pancreatic trypsin inhibitor terminate effect on cells. Haimeng study Industrial Co., integrity management, welcomed the new and old customers call to discuss! Contact: Zhou Li 021-61720900 / 60536060 13917028007/13917026856 QQ: 1317125719 Want: mengjiu2015