Haimeng study Industrial Co., Ltd. Supply maltase

The amount of detail Haimeng study Industrial Co., the manufacturer of high-quality, maltase phone manufacturers, CAS Number maltase, Shanghai maltase latest offer, maltase, maltase prices, maltose enzyme manufacturers latest offer, maltase , formula maltase, maltase molecular weight. Maltase maltase turned out to be for the name can generate two molecules of glucose, maltose hydrolysis enzyme used, but then generally acting as an alias with a sugar group in connection with various α-D- glucoside α- glucosidase to use. It can be cut from the non-reducing end oligosaccharide substrate α-1,4 glucosidic bonds, releasing glucose, or the liberated glucose residues in α-1,6 glycosidic bond to another substrate thus giving a non-fermented Isomaltooligosaccharide (IMO), a glycolipid or sugar peptides. α- glucosidase in the starch processing industry can be used glucoamylase preparations, together with α- amylase production of high dextrose syrup. In addition, α- glucosidase enzyme preparation as a key industrial production of highly valued IMO domestic and foreign food industry. Stage, α- glucosidase used in industrial production mainly from Aspergillus niger (Aspergillusniger). On a strain isolated from deep-sea hydrothermal vents ultra-thermophilic archaea (ThermococcussiculiHJ21) strains were studied to optimize production and enzymatic properties of α- glucosidase Condition. Intracellular fermentation time was 6h, an extracellular fermentation time was 21h, more suitable for producing α- glucosidase; the optimum temperature for the enzyme 85 ℃, pH of 6.5, NaCl concentration of 2.5%; soluble starch, yeast and peptone promote enzyme production. The α- glucosidase optimum temperature was 100 ℃, 90 ℃ half-life of 2h; optimum enzyme pH 7.0, the enzyme activity at pH between 5.0 to 8.0 is relatively stable; do not rely on the thermal stability of the enzyme Ca2 +, and Ca2 + inhibited the enzyme, metal ion Cu2 +, Al3 +, Ni2 + have a more significant inhibition of the enzyme, Hg2 + strongly inhibited the enzyme activity, and EDTA, Fe3 +, and K + could activate the enzyme. In T.siculiHJ21 chromosomal DNA as a template, using PCR technology, successfully cloned α- glucosidase gene T.siculiHJ21 of. Gene sequences showed that the gene open reading frame size of 729bp, encoding a protein molecule containing 242 amino acid residues, molecular weight of about 27.2kDa. Α- glucosidase enzyme gene sequence with Genbank database disclosed homology comparison analysis showed that the gene sequence T.siculiHJ21 of α- glucosidase and Thermococcushydrothermalis of α- glucosidase closest kinship with derived 81% amino acid homology of 90%. Haimeng study Industrial Co., integrity management, welcomed the new and old customers call to discuss! Contact: Zhou Li 021-61720900 / 60536060 13917028007/13917026856 QQ: 1317125719 Want: mengjiu2015