Furaltadone metabolites residues immune colloidal gold rapid detection

Summary: furaltadone metabolites residues immune colloidal gold rapid detection of major livestock organization is to detect whether it contains furaltadone metabolite (AMOZ) residues, the entire testing process takes about 90min, the product detection limit of 1.0μg / kg (ppb). Detection accuracy is high, simple and convenient operation. Specifications: furaltadone metabolites residues immune colloidal gold rapid detection reagent (40 plates / box) contains: Reagent board, dropper, desiccant AMOZ complex solution (dark green label) 22mL × 1 bottle AMOZ derivatization reagent 1,2 (reddish brown label) for each bottle 5mL × 1 1 M hydrochloric acid (purple label) 30mL × 1 bottle of 0.1 M dipotassium hydrogen phosphate (red label) 60mL × 5 5mL bottles centrifuge tube (40 / box) 50mL centrifuge tubes (40 / box) 1 M NaOH (green label) 20mL × 1 bottle long shift droplets tube (40 / box) Manual (1 / box) sample processing: 1. to take a certain amount of adipose tissue samples, with a mean minced uniform quality machine; 2. Weigh 2g homogeneous sample 50mL centrifuge tube and add 4mL of deionized water, 0.5mL 1M hydrochloric acid, 0.1mL AMOZ derivative reagent 1, 0.1mL AMOZ derivative reagent 2, mixed 3min ; 3. 60 ℃ water bath at incubation 60 min; was added 5mL 0.1M dipotassium hydrogen phosphate 4. After removal, 0.4mL 1M sodium hydroxide was added 6mL AMOZ extractant (ethyl acetate), mixing 1min, 4000rpm, centrifugation 5min; 5. shift droplets with a long tube pipette 5mL upper solution in the centrifuge tube 3mL, 65 ℃ air dry; 6. to dry the centrifuge tube was added 1mL AMOZ scavenger (n-hexane), stamped oscillation 1min, then adding 0.3mL AMOZ complex solution, upside-down mixing, 4000 rpm, centrifugal 1min (or standing to clear stratification); 7. draw 100μL sample solution to be tested in the pores of the gold standard, with a dropper to percussion hole red substance was completely dissolved, the reaction to wait five minutes;