Farm organizations furazolidone metabolite detector

Farm organizations furazolidone metabolite detector user groups: 1. Ministry, provincial, city, county, town agricultural testing centers at all levels; 2. The regional central slaughtering centers; 3. The competent authorities at all levels: the agricultural sector, the business sector, Food and Drug Administration and the like; 4 large batch of meat market, large-scale pig breeding base; 5 large fresh market, supermarket; 6. meat processing enterprises. Furazolidone belong nitrofuran antibacterial drugs, because of its low cost high efficiency, widely used in livestock and aquaculture. Furazolidone original drug rapidly metabolized in vivo, in vivo metabolic soon as 3-amino-2-oxazolidinone, metabolites can be long. Over the years studies have shown that furazolidone metabolite has a strong carcinogenic, teratogenic, mutagenic effect. Furazolidone metabolite residues in animal foods pose a threat to human health, resulting in the world prohibited. Farming of the Ministry of Agriculture document [2002] No. 1 is also provision of food of animal origin furazolidone detection limit is not detected. Farm organization of the company developed the furazolidone metabolite detector is used for rapid detection of animal tissue furazolidone metabolite (AOZ) residues, the entire testing process takes about 90min, the product detection limit of 0.5μg / kg (ppb). At the same time the use of this product and furazolidone metabolites by liquid chromatography - tandem mass spectrometry method of 192 samples (103 negative samples and 89 positive samples) were detected, the test results show that the product liquid chromatography - tandem mass spectrometry method results in line with the rate of 99.4%. Farm organizations furazolidone metabolite detector Specifications: Livestock organization furazolidone metabolite detector (40 plates / box) contains: Reagent board, dropper, desiccant AOZ complex solution (dark green label) 22mL × 1 bottle AOZ derivatives reagent (reddish brown label) 5mL × 1 bottle of 1 M hydrochloric acid (purple label) 30mL × 1 bottle of 0.1M dipotassium hydrogen phosphate (red label) 60mL × 5 bottles of 1 M NaOH (green label) 20mL × 1 bottle 5mL centrifuge tubes (40 / box) 50mL centrifuge tube (40 / box) long shift droplets tube (40 / box) Manual (1 / box) poultry tissues furazolidone metabolite detector sample processing: 1. Take to a certain amount of fat tissue samples, using a homogenizer to crush even; 2. Weigh 2g homogeneous sample in 50mL centrifuge tube and add 4mL of deionized water, 0.5mL 1M hydrochloric acid, 0.1mL AOZ derivatization full mixing 3 min; 3. 60 ℃ water bath at incubation 60min; 4. Remove the plus 5mL 0.1M potassium phosphate dibasic, 0.4mL 1M sodium hydroxide was added 6mL AOZ extraction solvent (ethyl acetate), mixed 1 min, 4000rpm, centrifugal 5min; 5. shift droplets with a long tube pipette 5mL upper solution in the centrifuge tube 3mL, 65 ℃ air dry; 6. to dry the centrifuge tube was added 1mL AOZ scavenger (n-hexane). stamped oscillation 1 min, then add 0.3mL AOZ complex solution, upside down mix, 4000rpm, centrifugal 1min (or standing to clear stratification); 7. draw the sample solution to be tested in the gold standard 100μL micropores, with drops pipetting tube into the hole on a red substance was completely dissolved, wait for 5 minutes; Farm organization furazolidone metabolite cross-reactivity detector: Species cross-reactivity rate (%) AOZ 100 AMOZ