Yeast β- glucan liquid chromatograph

Yeast β- glucan LC yeast glucan with β- β- (1,3) -D- glucan main chain, β- (1,6) branches of a polysaccharide which can be used contain Formula: (C6H10O6) n, 125≤n≤25000 relative molecular mass: 20kDa ~ 4000kDa (according to 2007 international atomic weight table) with reference to QB / T 4572-2013, applicable to the genus Saccharomyces cerevisiae as a raw material in recent (Saccharomyces sp.) over the cell wall, enzymatic hydrolysis, acid, alkali treatment, separation and purification, drying and packaging process to obtain the yeast β- glucans, which can be used as food and food ingredients. According to the statutory amount of glucose in the external standard between mobile and stationary phases have different distribution coefficients, the sample after hydrolysis into the liquid chromatography using a water mobile phase, after the carbohydrate molecules flowing past a differential detector, available Determination. Equipment vortex mixer performance liquid chromatograph: with refractive index detector and sugar column (6.5mmx300mm waters sugar pak-1 or equivalent analyze the effect of the separation column) chromatographic conditions using water as the mobile phase, a flow rate of 0.5ml / min, The column temperature was 80 ℃, the instrument is stable baseline backward like yeast β- glucan configure a high performance liquid chromatography: the pump. P501 1 set of Zhejiang differential refractive index detector RID-10A 1 set Shimadzu Chromatography Workstation N2000 1 set Zhejiang Syringe 100ul 1 set Shanghai manual injection valve 7725i 1 set 7725i column oven RPL-D2000 1 set Pulido sugar column Num: WAT085188, SUGAR PAK I 6.5MMX300MM COL 1 set waters efficient yeast β- glucan LC configuration II: constant high pressure pump waters 515 infusion pump 1 set waters used differential refractive index detector waters 410 Refractive Index 1 set waters used chromatography workstation N2000 1 set of Zhejiang Syringe 100ul 1 set Shanghai Manual Injectors 7725i 1 set 7725i column oven RPL-D2000 1 set Pulido sugar column Num: WAT085188, SUGAR PAK I 6.5MMX300MM COL 1 set waters Keywords: Yeast β- glucan chromatograph, β- glucan chromatograph, yeast β- glucan HPLC