HPLC analysis of glyphosate and PMIDA

HPLC analysis of glyphosate and PMIDA HPLC column analysis with glyphosate and PMIDA Keywords glyphosate PMIDA quantitative HPLC high performance liquid [Abstract] Chromatography (GE-501 liquid chromatograph) to potassium dihydrogen phosphate buffer solution as the mobile phase, Vertex SAX column, a flow rate of 0.8mL / min, UV detector wavelength 195nm hereinafter glyphosate analysis column and dual Gan phosphine content. The results show that glyphosate and PMIDA linear correlation coefficient of 0.9999 and 0.9984, 0.0899 and 0.7701 standard deviation, coefficient of variation of 0.1620% and 0.9660%. Glyphosate is a suction-type organic phosphorus herbicide inside spread, with broad-spectrum, high efficiency, low toxicity, safety and other characteristics are widely used. Analysis of glyphosate mainly liquid chromatography and UV spectrophotometry breadth method. PMIDA is the preparation of intermediates of glyphosate, currently general quantitative analysis using titration. On the basis of experimental study on the establishment of quantitative analysis with the column by HPLC glyphosate and PMIDA two components were well separated under selected conditions. The method has higher precision and accuracy, simple, fast, get the ideal application effect research project air (oxygen) oxidizing PMIDA system of glyphosate. 1 Experimental 1.1 Reagents and solutions freshly distilled twice-distilled water, methanol (HPLC grade), potassium dihydrogen phosphate (AR). Glyphosate and PMIDA Standard: content> 99.5%. 1.2 Instrument GE-501 liquid chromatograph: a detector GE-501 UV detector, GE-501 high pressure constant flow pump, Vertex SAX 4.6x300mm glyphosate applicable detect columns, filters (filter pore size 0.45um), ultrasound cleaner 2L. 1.3 Chromatographic conditions Mobile phase: 0.001mol / L potassium dihydrogen phosphate solution, adjusting the PH value of 2.2 with phosphoric acid, filtered, degassed ultrasound. Six Degrees of 0.8ml / min, detection wavelength 195nm, column temperature: room temperature, injection volume 20ul. Glyphosate bit retention time 6min, PMIDA bit 10min. 1.4 Measurement procedure 1.4.1 Standard Preparation Weigh glyphosate standard of about 0.1g, PMIDA standard 0.05g, as for 50ml volumetric flask, dilute with mobile phase to dissolve mark and shake. 1.4.2 Preparation of test solution Weigh sample amount, as for 50ml volumetric flask, dissolved in mobile phase diluted to the mark. 1.4.3 measured by the above operating conditions, after the instrument is stable, continuous injection of several doses of the standard solution, knowing the adjacent 2-pin standard peak area relative changes of less than 1.5%, the installation standard solution, sample solution, the test sample solution, standard solution were measured sequentially, chromatography shown in Figure 1. The above method is also applicable to the production of glyphosate use and PMIDA production enterprises in quality control of the production of glyphosate content from (Hangzhou tournaments Technology Co., www.htcee.com)