Japan YMC-Pack phenyl column

YMC-Pack phenyl column from single phenyl bonded phase fully terminated. Stable mechanical properties. Bonded to the silica carrier 120A and 300A. Single phenyl fully capped priority reservations compound aromatic ring. ODS and C4 can be replaced in the analysis of peptides and other biomolecules. In analyzing the column size or preparation may be used 120A, 200A and 300A pore size. Particle size 3,5, 10,15μ pore size: 120A and 300A carbon content: 9% and 3% long column life YMC phenyl phase is a high density of bonded phases. On silica gel 120A 9% carbon content. Two steps terminated. Creating a greater bond relatively phenyl reverse phase column, there are exceptionally long life. Selectivity in comparison with a uniform linear aliphatic reverse phase column, e.g., as compared to C18, C8 and C4, have π electrons increases with the relative retention phenyl ring aromatic analytes. Phenyl analysis can replace ODS and C4 peptides and proteins. Holes 120A and 300A macroporous silica support can be separated. A relatively small hole in the big hole phenyl phenyl phase 120A retention decreased. Typically the pore size increases surface area decrease, resulting in shorter retention times. Specification phenyl bonded phase average pore size of 120A 300 135 ± 15A 300 ± 40A surface area m2 / g 300 ± 40 100 ± 20 pore volume mL / g 1.0 ± 0.10 0.9 ± 0.15 carbon content of 9% 3% Application Data Polyphenol 1 .Pyrogallol2.Hydroquinone3.Catechol4.Phenol H890627A Column: YMC-Pack Ph (5μm, 120A) 250 X 4.6 mm IDEluent: 5mM acetic acidFlow rate: 1.0 mL / minTemperature: 25 ℃ Detection: UV at 280 nm, 0.32 AUFSSample: reagentsInjection : 20 μL Copyright YMC Co., Ltd. Defferent selectivity between Ph and ODS 1.p-Aminobenzoic acid (PABA) 2.Biotin (Vitamin H) 3.Riboflavin (Vitamin B2) 4.Cyanocobalamin (Vitamin B12) Column: 150 x 4.6mmI.D.Eluent: acetonitrile / 50mM NH4H2PO4 (10/90) Flow rate: 1.0mL / minTemperature: 37 Detection: UV at 210nm, 0.16AUFSInjection: 10μL (0.02-0.30mg / mL) This figure shows that the ODS and benzene pillar separating the retention time difference when a water-soluble vitamin.