Deer EHF rapid test strip

REAGEN? Deer EHF rapid test strip RNS92036 I. Introduction 1. Kit Description:? REAGEN deer epidemic hemorrhagic fever (EHDV) test strip is a rapid and sensitive method to detect deer epidemic hemorrhagic fever virus (EHDV). Deer epidemic hemorrhagic fever virus (EHDV) is a specialized infection of wild and domestic ruminants circovirus, epidemic hemorrhagic fever (EHD) exist in many countries, especially white-tailed deer in North America, the morbidity and mortality up to 90%. Deer, elk and moose can produce antibodies. Acute onset, can be found edema, bleeding and ulcers in the mouth and lips of infected animals in. Chronic disease, infected animals showed the hoof wall off, erosion and rumen scars. Spontaneous abortion and stillbirth also appear in some epidemiological report. In the wild ruminants, viral infection can last 15-40 days. Usually 6 days after infection, the experimental animals maximum virus (titer> 106 pfu / ml). REAGEN? Deer epidemic hemorrhagic fever (EHDV) test strip is a rapid, qualitative methods to detect clinical samples (serum, plasma) deer epidemic hemorrhagic fever virus (EHDV). EHDV strip surface proteins by using a monoclonal antibody, to increase detection sensitivity. The method can accurately detect EHDV, no cross-reactivity with BTV. The test strip features:? Rapid detection methods ---- 7 minutes the result special serum, plasma EHDV specific monoclonal antibody with high sensitivity, high reproducibility 2???. The kit works: The method is based on a competitive technology GICA serum sample to be tested 200uL added to the transparent plastic strip cards or tube wells, and then allow the sample to be coated pink lyophilization bottom of the hole powder dissolves. Timing 3 minutes, so that the gold standard ion present in the sample with EHDV binding. The test strip card inserted in the arrow direction perpendicular to the sample wells of the sample flow test strip. All the samples did not EHDV conjugated gold particles will be combined with standard anti-gold standard ion antibody test line (T- line) on. Forming a red tag line at the test line position. If the gold-labeled antibody and sample EHDV combined, the gold standard will flow through the test line (T- line) to the control line (C- line). Visual determination result, if the test line (T- line) color significantly darker than the control line (C- Line) was negative. Test line (T- line) color equivalent to or significantly weaker than the control line (C- line) indicates the sample contains EHDV. Test line (T- lines) color than the control line (C- Line) obviously a lot of light, then the sample is high EHDV content. 3. Kit components and storage:? The REAGEN deer epidemic hemorrhagic fever (EHDV) test strip containing 24 test holes (RNS92036-01) or 48 test holes (RNS92036-02). Save the shelf life of one year. Number kit consisting of (RNS92036-01) Number (RNS92036-02) storage conditions wells 1x 24 bar (total 24) 2x 24 bar (total 48) 4 ° C strip 1x 24 bar (total 24) 2x 24 bar ( a total of 48) serum dilution 20ml 40ml 4 ° C tip 48 96 room Each kit provides a 96-well plastic plate frame to use for testing, if needed can be reused.