Phenylethanolamine A rapid test kit

I. Summary phenylethanolamine A (Ke Lunba amine) is a new generation of clenbuterol is a stimulant that is adrenoceptor agonist. In the late 1980s found that promote the growth of livestock, reduce fat, increase lean meat in effect, it has been used as feed additives. However, since the early 1990s, it appears more than phenylethanolamine A poisoning, and the emergence of deaths, it is prohibited. Phenylethanolamine A rapid detection kit uses one-step direct competitive ELISA can urine, meat tissue phenylethanolamine A rapid quantitative detection. Second, the principle of the reagent kit uses an indirect competitive ELISA assay urine samples, tissue and the like phenylethanolamine A, the kit by the coupling antigen pre-coated microtiter plate, horseradish enzyme markers, antibodies, standards and Other reagents composition. Detection, added to the standard or sample solution, sample phenylethanolamine A microtiter plate and pre-coated with anti-benzene ethanolamine A antibody conjugate antigen competition, after adding the enzyme markers, with TMB chromogenic substrate sample absorbance A benzene ethanolamine and its content was negatively correlated with the standard curve can be derived residues in samples of phenylethanolamine A. Third, the use phenylethanolamine A test kit can be qualitative and quantitative detection of animal tissue and urine samples of phenylethanolamine A residual amount. Fourth, technical indicators 1 kit sensitivity: 0.05ppb (ng / ml) 2 reaction mode: 25 ℃, 30min ~ 15min. 3 Sample Recovery: urine: 95% ± 10% tissue: 85% ± 15% V. Kit components ELISA plate, standard solution, high standard solution, the enzyme-labeled substance, antibody working solution, substrate solution A, bottom was liquid B, stop solution, 20X concentrated cleaning solution, 10X six complex solution, storage and shelf storage conditions: The kit stored at 2-8 ℃, do not freeze. Shelf life: This product is valid for one year, the production date see packaging. First, before a urine sample processing approach: direct access to the clear urine were measured. 2 tissue sample: weigh the homogenized tissue samples taken, adding complex solution, sufficiently shaken at room temperature centrifuged and the supernatant was analyzed. Second, the enzyme-linked immunosorbent assay reagents required steps removed from at 4 ℃ environment, kept at room temperature for more than 30min. 1 Number: The samples and standards corresponding to the pores sequentially numbered, each sample and standard in duplicate holes. 2 sample reaction: Add standard or sample to the respective wells, and enzyme markers, then add the antibody working solution, mixing, reaction 25 ℃ 30 minutes. 3 wash: the hole liquid drying, washing work washed five times, pat dry with absorbent paper. 4 color: each hole by adding substrate solution A, substrate solution B, mixing, 25 ℃ dark color for 15 minutes. 5 Termination: adding the Stop Solution, mix, stop the reaction. 6 measuring absorbance: measured with a microplate reader. 1 ppb standard or sample light to calculate the rate ppb standard or sample rate equal to the average percentage of the absorbance values ??(holes) by the first standard (0ppb) absorbance value, multiplied by 100%, i.e. the percentage absorbance value (%) = A × 100% A0 A- average absorbance of the standard solution or sample solution absorbance value A0-0ppb average value of standard solution 2, and the calculation of the standard curve plotted with the standard percentile absorbance of ordinate corresponding standard concentration (ppb) of logarithmic abscissa, the semi-logarithmic graph standard. The percentage of samples absorbance standard curve, the concentration of the sample is read from the corresponding standard curve, multiplied by the actual concentration of the corresponding dilution of the analyte in the sample is. If the ELISA kits special software has been developed, but for exact and rapid analysis. (Welcome to call to obtain)