DON rapid detection card

First, the product uses and principles of the product for the detection of grain, feed samples DON residues. This product employs the competitive inhibition GICA principle, when the sample solution is dropped into the sample well, the sample solution and gold-labeled antibody DON combine prevent gold-labeled antibody and a cellulose membrane binding DON conjugates . When the sample solution DON content is greater than the detection limit of the test line is not the color, the result is positive; when the sample solution vomitoxin content is less than the detection limit of the test line was purple, the result was negative. Second, the packaging composition 1. Individually wrapped rapid detection card (containing test card, disposable dropper and a desiccant one each) 50 / box 2. Manual 1 / box Third, technical indicators 1, the kit sensitivity: 30μg / kg (30ppb) 2, the lowest detection limit: grain, feed: 30ppb Fourth, preservation and valid at room temperature (2 ℃ -30 ℃), dry, dark place, valid for 12 months. [Need to use but does not provide reagents, instruments] analytically pure methanol, 1.5 / 15ml centrifuge tubes, heterogeneous machines, scales, centrifuges, pipette steps a, sample processing 1. grains, feed sample was pulverized to through 20 mesh sieve (particle size and relatively instant coffee); 2. Weigh 1g good crush the sample in 15ml centrifuge tubes, add 5ml sample extract (1: 9 methanol: water), shaking for 15 minutes; 3. The temperature at 4000r / min centrifugal 5min, or stand for 10 minutes; 4. the supernatant solution to be tested. Second, the use to read in its entirety before the test manual, before using the test card, reagent and sample solution to be tested to room temperature (20 ℃ ~ 30 ℃). 1. Remove the bag from the original reagent plate, placed horizontally on the front of the viewer, on the right as shown above (Please use immediately after opening); 2. draw the tested solution with a dropper, to sample hole in the vertical drop Add 3 drops (approximately 75μl), after sample start time; 3. The results should be read at 5-8 minutes. First, the result of judgment 1. Negative: observation hole, only the control line region (C) appears a purple line. 2. Positive: In the observation hole, test line region (T) and control line region (C) simultaneously purple line. 3. Failure: In the observation hole, the control line region (C) does not appear the color line. Second, note 1. Because of the failure to detect the card easily damp, individually wrapped in a foil bag test card using the front can be opened. 2. Do not touch the white membrane surface center of a test card.