Rapid detection of aflatoxin B1 card

First, the product uses and principles of the product for the detection of grains, feed and other samples of aflatoxin B1 residues. This product employs the competitive inhibition principle GICA, when the sample solution is dropped sample well, the sample solution of aflatoxin B1 and gold-labeled antibody combination, prevent gold-labeled antibody and a cellulose membrane aflatoxin B1 conjugate binding. When the sample solution of aflatoxin B1 content is not larger than the detection limit of the test line color, the result is positive; when the sample solution of aflatoxin B1 content of less than detection limit was purple test line, the result was negative. Second, the packaging composition 1. Individually wrapped rapid detection card (containing test card, disposable dropper and a desiccant one each) 50 / box 2. Manual 1 / box Third, technical indicators 1, the kit sensitivity: 5μg / kg (5ppb) 2, the lowest detection limit: grain, feed: 5ppb Fourth, preservation and valid at room temperature (2 ℃ -30 ℃), dry, dark place, valid for 12 months. [Need to use but does not provide reagents, analytical instruments] ethyl acetate, 1.5 / 15ml centrifuge tubes, heterogeneous machines, scales, centrifuges, pipette steps a, sample processing 1. grains, feed and other samples It can be crushed to 20 mesh sieve (particle size and relatively instant coffee); 2. Weigh 1g good crush the sample in 15ml centrifuge tubes, add 5ml 2.5ml deionized water and ethyl acetate, sealing, shock 5 minutes ; 3. The ambient temperature at 4000r / min centrifugal 5min, the supernatant set 1ml glass tube, nitrogen blowing instrument dry; 4. Limit the table plus the amount of sample dilution according to the required testing, fully shock dissolved in a glass test tube and dried The residue, this solution is test solution. (Turbid liquid has dissolved after if required 4000r / min centrifugal 5min, the supernatant tested) Second, use first read the instructions before complete testing prior to use recovery test card, reagents and sample solution to be tested to room temperature (20 ℃ ~ 30 ℃). 1. Remove the bag from the original reagent plate, placed horizontally on the front of the viewer, on the right as shown above (Please use immediately after opening); 2. draw the tested solution with a dropper, to sample hole in the vertical drop Add 3 drops (approximately 75μl), after sample start time; 3. The results should be read at 5-8 minutes. First, the results to determine the negative (-): T line color (test line, near the loading aperture end) color, indicating that the sample aflatoxin B1 concentrations below detection limit or no aflatoxin B1. Positive (+): T line no color, indicating that the samples above the detection limit of aflatoxin B1. Invalid: C line does not appear, it may operate improperly or reagent card has expired. You should read the instructions again and re-test with a new reagent card. Second, note 1. Because of the failure to detect the card easily damp, individually wrapped in a foil bag test card using the front can be opened. 2. Do not touch the white membrane surface center of a test card. 3. Take the droplet tube can not be mixed, to avoid cross-contamination. The sample solution to be tested need clear, no turbidity particles, no bacterial contamination, or likely to cause obstruction, and abnormal color is not obvious, and thus determine the impact of the experimental results.