Sheep Peste des petits ruminants virus antibody test card

First, the principle of the reagent test card using GICA test (GICA) principle made after the sample is added to the sample well with colloidal gold chromatographic membrane markers move along together, if there PPRV makes detection of antibodies in the sample line show purple, if the sample does not exist PPRV antibody response is not generated color. Second, the scope commonly known as sheep peste des petits ruminants plague, rinderpest caused by pseudo peste des petits ruminants virus (Peste des Petits Ruminants Virus, PPRV) goats, sheep, wild small ruminants highly contagious animal diseases. The reagents for the detection of small goat serum PPR virus antibodies that can be used sheep PPR infection diagnosis, Vaccine Evaluation. Third, Packing: 50T / box Fourth, storage and shelf life stored in a cool, dry place (2-30 ℃), the shelf life of 24 months. A serum sample preparation steps: 2-3ml blood extracted by conventional methods set clean, dry test tube and allowed to stand for about 1 hour until the blood coagulation after 4000 rev / min and centrifuged for 10 minutes (can also be blood to stand for about 2 hours until the blood clotting natural precipitation serum), serum was separated. Requiring serum clear, no hemolysis, no pollution. Serum samples can be stored in short-term 2 ~ 8 ℃, long-term need is set at -20 ℃. Whole Blood: whole blood can be used as an anticoagulant test sample (whole blood samples should not be frozen). If not detected immediately dropped after anticoagulant Calgary wells shall be taken out for testing, so as not to affect the flow of the sample solidification can not experiment. Second, the operating method of the hole in the sample test card add 2 drops (approximately 80ul) to be detected in serum samples. 2. The test card flat on the table, to stand at room temperature for 5-20 minutes determination result, more than 20 minutes of the results only as a reference. Third, please read the instructions carefully Cautions 1. Detection, this product provides a variety of reagents for this experiment; 2. The test card should be removed from the refrigerator to return to room temperature, open, open the test card should be used as soon as possible in order to avoid After moisture test card will expire; 3. Serum samples were subject should be fresh and clear, hemolysis or corrupt sample is available for testing; 4. Experiment pollutant waste disposal regulations shall be handled properly. [Results] 1. The negative judgment: the observation hole, only the control line region (C) appears a purple line. 2. Positive: In the observation hole, test line region (T) and control line region (C) simultaneously purple line. The higher the antibody titer test line (T) the darker. 3. Failure: In the observation hole, the control line region (C) does not appear purple line. Interpretation of test results [1] If you are not vaccinated Peste des petits ruminants vaccine: 1.1 when the subject sample test line (T) at no ribbon, suggesting that the test sample was not Peste des petits ruminants virus antibodies. If appropriate acute symptoms, it can not be ruled out Peste des petits ruminants virus infection. 1.2 When the subject sample test line (T) appears at the obvious ribbon, it is possible for the wild virus infection or past infection, requires a combination of clinical and other methods of analysis. 2 If you have vaccinated Peste des petits ruminants vaccine: 2.1 when the subject sample test line (T) strip of color control card ≥ 1:40 titers, indicating a higher antibody titer, has reached the level of protection. 2.2 When the subject sample test line (T) strip color