Canine distemper virus antigen detection cards

I. Summary of distemper often prevalent in a variety of carnivores, especially dogs more easily through the respiratory system infections. Because the disease is difficult to cure, so the higher mortality rate. Dogs infected with canine distemper virus (CDV) can check for about one week after the virus in the blood, fever, dry cough for about two weeks after infection, conjunctivitis, vomiting, diarrhea and other symptoms. Second, the principle of canine distemper rapid antigen test card test card is a quick immunochromatographic detection technique for detection of canine distemper virus antigen. After the sample is added to the sample hole, the colloidal gold-labeled monoclonal antibody anti-CDV move together along the chromatographic membrane. If the presence of CDV antigen in the sample, and antibody binding assays line is displayed in red wine. If the sample does not exist in CDV antigen, no color-producing reaction. Third, the scope: Products for hot dogs viral antigen detection for disease diagnosis. Fourth, the reagent composition 1. canine distemper virus antigen detection card 15 2. Sample Diluent 15 bottles 3. Cotton 15 V, storage and shelf life stored in a cool dry place (2-30 ℃), the shelf life of 24 months. Swabbing conjunctival secretions [Steps] First, the operating method of a cotton swab to draw just discharged urine or swab soaked with saline after. 2. Immediately insert the swab containing the sample dilution of the sample tube, swab samples were sufficiently stirred until the swab is all into dilution after the swab in the tube wall squeezed discarded sample was allowed to stand aside. 3. The serum can be collected using a dropper that comes with the drops of the serum sample containing the sample dilution dropped sample tube and mix well spare. The test card from the sealed bag out flat on a dry surface, supernatant samples were taken from the sample tube and slowly dropped 5 drops (approximately 80μl) to detect card sample wells. 5. determination results within 10-15 minutes at room temperature, the result of more than 15 minutes only as a reference. Second, note 1. The test strips and reagent usually stored in the refrigerator, remove from the refrigerator before use, return to room temperature after use; 2. The test card should be used immediately after opening the bag, avoid moisture absorption failure; 3. Sample collection directly affect the accuracy of the test results, should ensure proper and adequate; 4. excess manure may lead to erroneous results (black bands appear), can dilute the sample diluted before measurement. Sample dilution 5. Different products can not be mixed, aluminum foil bags damaged product can not be used. 6. Experiment pollutant waste disposal regulations shall be handled properly.