Rabies virus antigen detection cards

I. Summary of rabies that is rabies, also known as hydrophobia, is a violation of acute viral infectious disease of the central nervous system, all warm-blooded animals, including humans, are likely to be infected. It is more from the infected animal bite derived. It is generally believed that a white foam lip mad dog bite infection, in fact, cats, ferrets, raccoons, skunks, foxes or bats may be sick and contagious. Second, the reagent card principle rabies virus (RBV) antigen test card using the fast immunochromatographic detection technology for detecting cat, dog saliva secretions rabies virus antigen. After the sample to be tested is added to the sample hole, and colloidal gold-labeled monoclonal antibody anti RBV chromatographic membrane to move along together. If the presence of RBV antigen in the sample, and antibody binding assays line is displayed in red wine. If the sample does not exist RBV antigen, no color-producing reaction. Third, the scope: This product is mainly used to detect cats, dogs and other animals, the rabies virus antigen in saliva secretion virus, suitable for disease diagnosis. Fourth, the reagent composition 1. canine parvovirus antigen detection card 25 2. Sample Diluent 25 bottles 3. Cotton 25 V, storage and shelf life stored in a cool dry place (2-30 ℃), the shelf life of 24 months. [Steps] First, the operating method of a cotton swab to collect saliva. 2. Immediately insert the swab containing the sample dilution of the sample tube, swab samples were sufficiently stirred until the swab is all into dilution after the swab in the tube wall squeezed discarded sample was allowed to stand aside. 3. The test card from the sealed bag out flat on a dry surface, supernatant samples were taken from the sample tube and slowly dropped 5 drops (approximately 80μl) to detect card sample wells. 4. determination result within 10 to 15 minutes at room temperature, the result of more than 15 minutes only as a reference. Second, note 1. The test strips and reagent usually stored in the refrigerator, remove from the refrigerator before use, return to room temperature after use; 2. The test card should be used immediately after opening the bag, avoid moisture absorption failure; 3. Sample collection directly affect the accuracy of the test results, should ensure proper and adequate; 4. excess manure may lead to erroneous results (black bands appear), can dilute the sample diluted before measurement. Sample dilution 5. Different products can not be mixed, aluminum foil bags damaged product can not be used. 6. Experiment pollutant waste disposal regulations shall be handled properly. [Results] 1. The negative judgment: the observation hole, only the control line region (C) appears a purple line. 2. Positive: In the observation hole, test line region (T) and control line region (C) simultaneously purple line. 3. Failure: In the observation hole, the control line region (C) does not appear the color line.