Capillary Column

Description: Install a Capillary Column as follows: Step 1: Check gas filter, carrier gas, injection pads and liners and other tests septum and gas filters, auxiliary gas, and a detector to ensure smooth and effective gas. If done before a higher boiling point compounds, it requires the injection port liner cleaned or replaced. Step 2: Set the nut and the column, and the column carefully cut flat at one end of the column is mounted on a corresponding nut and ferrule, no support this time around of the points column port, two-port column bracket section should always be towards the oven door. After installing the nut and ferrule, the column port cut flat, and with a magnifying glass to check to make sure the incision and the wall at right angles, and no residual debris, no cutting edges or uneven surfaces. Step 3: The column is connected to the port injector Generally speaking, the top of the column should be maintained in the lower inlet liner, when the syringe is fully inserted through the septum inlet, if the tip and the substrate the difference between the top of the column tube 1-2cm, this is the ideal state. Remove sufficient length to be connected from the column rack, follow the steps to cut the column 2, connected to the inlet. Avoid pressing hard curved capillary column, and be careful not to let signs and other items with sharp edges in contact with the column friction to prevent the pillar broken or damaged. After the column correctly embedded in the inlet, connect the hand nut, hand tighten with a wrench and then tighten after 1 / 4-1 / 2 turn. Step 4: Turn the carrier gas must be high purity nitrogen carrier gas (or helium), a purity of 99.999%, the use of polar column (eg FFAP, PEG-20M, etc.), the best-deoxy-tube carrier gas plus a further oxygen, This extends the life of the column. When the column to the inlet connection, connect to the carrier gas. Adjusting the pressure to get the right anterior column carrier gas flow rate. Connect the other end of the column (dead-end), insert the vial containing hexane, under normal circumstances, we can see the bottle steady and continuous bubbles. If no bubbles, it is necessary to re-examine a download gas installations and flow controllers are installed completely or settings are correct and detect what the whole gas line leaks. Once all the issues resolved, the column port removed from the bottle, wipe clean to ensure no solvent residue column port, then the next step to install. Capillary pressure before the reference set (Mpa) column length m column diameter (mm) 0.25 0.32 0.53 15 0.05 0.04 0.02 30 0.1 0.08 0.03 50 0.18 0.14 0.05 Step 5: The column is connected to the detector to install and required attention of Matters relating to the above column and the inlet connection (Step 3) is substantially the same as described in the section. Note that when you install the column end points higher than the makeup, or installed in accordance with the instructions chromatograph. Step 6: the gas leak before the column was heated to the GC system for leaks. 1:1 recommend using a mixed solution of isopropanol / water to be checked. Step 7: Column after column of aging installation and system leak detection work is completed, the column can be aging. The column temperature program to the maximum operating temperature below 30 ℃, constant temperature for 30 minutes. Component adsorbed within this column will be blown, so as not to affect your analysis. Finally, the column temperature was lowered to the desired temperature, actual samples can be analyzed.