Nm laser optical tweezers Analyzer

Within five minutes to upgrade microscope nm laser optical tweezers analyzer NanoTweezer new nano-optical tweezers conversion device is a microscope attached devices. The device allows researchers to use existing microscopes to capture, manipulate nanoscale particles. NanoTweezer new nano-optical tweezers conversion device, using the world's advanced integrated optical waveguides and resonant body, laser emitted by the micro-chip capture and manipulate nanometer to micron-sized clump of particles. Can achieve a variety of applications, such as the operation is much less than conventional optical tweezers sample and keep particle structure is not destroyed; implementation of new types of experiments and analysis of the particle size range captured manipulation: 10nm-5 micron sensitivity (Sensitivity):. 10650.3V / W power sensitivity (Power Sensitivity): 6 uW-12 mW laser wavelength (Wavelength): 1065nm laser power (Optical Power): 0-500 mW continuously adjustable optical interface: FC / APC SMPM optical isolation (Optical Isolation) : 33-38 dB integrate easily with existing microscope:? NanoTweeze easily research inverted microscope with international brands such as Zeiss, Nikon and Olympus combination. Stability is crucial:? Long term stability without manual adjustment and operational performance is NanoTweeze a key design principles. High stability of the laser light source and the optical path combining short reentry drift compensation designed to protect the NanoTweeze high performance test Applications include:? 1) analysis of protein aggregates) single particle spectrum (including Raman spectroscopy), 2) coating measurement 3) Shape Analysis 4) pigment analysis 5) sub-micron imaging Label Free Nanoparticle Sizing and Imaging Nanoparticle Functionalization and Coating Analysis Nanophotonics Based Optical Tweezing - Smallest Particles Ever Overview:! NanoTweezer new laser tweezers system is equipped with powerful optical capture system, manipulating objects covering single cells, single molecules, organelles, viruses, nucleic acids, metal nanoparticles, carbon nanotubes, protein. Easy operation is much smaller than conventional optical tweezers sample and keep the particle structure is not destroyed; the introduction of new types of tests and analysis; avoid surface chemistry; creating new nanostructures; retain the respect of the basic biological molecules, changing the background solutions; capture single bacteria, and observe its division and the like. NanoTweezer laser controller, and the special design of the optical resonator chip microscope adapter, directly seamlessly with existing microscope equipment. Features and highlights: 1) no damage of optical tweezers to manipulate biological particles in a gentle way to complete a non-mechanical contact gripping and manipulating objects, trapping force is exerted on the entire particle, non-mechanical capture as concentrated in a small area on, will not cause mechanical damage and pollution trapped biological particles. 2) do not interfere with the surrounding environment and invisible biological particles and penetrating its normal life activities of light, optical tweezers can be maintained in the case of living cells in the natural environment and its capture all the mechanical components of optical tweezers to manipulate objects from capture The distances are much larger than the capture object scales (1000), is a remote control 1) simple, strong ability to manipulate capture observed higher resolution: system acquisition ability to maneuver: a new generation of nano-laser tweezers system, using the new integrated optics photon resonance technology, capable of nanometer to micron-sized particles easily operate and capture manipulation of trapped particles size range: 10nm-5 micron; observation of biological molecules can also enhance the resolution, capturing bacterium observer splitting process. Observations higher resolution and high spatial resolution optical tweezers technology combine to make it have the ability to distinguish fine structures and the ability to dynamically control and function of particle types captured manipulation: A) biological material, such as protein aggregates, protein crystals, Antibodies with microtubules etc; B) nano-materials, such as quantum dots, carbon nanotubes, polymer beads, nano-silicon, titanium dioxide and the like. C) reversible nanoscale single cells, viruses, nucleic acids, nanoparticles, nanotubes and protein operations 2) is superior to conventional optical tweezers system: This system uses a chip-based photonic resonance capture technology, can achieve a variety of applications, The operation is much smaller than conventional optical tweezers sample and keep the particle structure is not destroyed; ordinary optical tweezers can capture and processing of 100 nm and smaller objects; the system by using the latest technology integrated photonic overcome obstacles scattered light, The optical resonator of this system may be enhanced by the strength of the produced optical waveguide gradient. Due to the concentration of the stronger points of light, you can manipulate the maximum reach of 1064nm particles. 3) the system online capability: with scientific-grade microscope combined with upright; with a laser microscope combined with Raman spectroscopy; Typical applications: - Accurate capture particles and traction particles are the most basic functions of optical tweezers optical tweezers trapped particles several nanometers to several microns, in this scale, it provides a means to study the microscopic mechanism of macroscopic phenomena, in particular for the study of biological cells to macromolecules from nano-biology, providing in vivo study conditions, such as laser Ease of optical tweezers to manipulate cells, can effectively separate the various cell organelles, and in the case does not substantially impact on the environment nondestructive catch live action. By capturing and separating cells, can understand many of the characteristics of cells, such as cell-cell adhesion, cell membrane elasticity, resilience and cell physiological processes and other cells, which studies real physiological processes of cells. 1. The field of application of the single cell 1.1 Traction capture nanoscale particles (cells capture and separation) laser with a wavelength of 1064 nm the aggregates moved nearly half a meter, and magnetic methods commonly used in the past, only the aggregates move a short distance. The new nm laser optical tweezers to manipulate the size range of particles captured: 10nm-5 micron, optical tweezers can be easily manipulated cells, can effectively separate the various cell organelles, and in the case does not substantially impact on the environment nondestructive catch live action. Through the capture and separation of cells able to understand many features of cells such as cell-cell adhesion, cell membrane flexibility, adaptability and physiological processes of cells, such as cell fusion, so as to effectively understand the true physiological process cells. 1.2 Cell resilience resilience inside the cell under normal circumstances is difficult to observe with a microscope. The optical tweezers can be for living cells non-invasive microscopic manipulation, can induce cells to produce strain elasticity of the elastic erythrocyte 1.3 measuring red cell membrane is the physiological function index of blood, measuring the red cell membrane elasticity technique, double optical tweezers method is the most direct and accurate way. 1.4 the optical tweezers to promote cell fusion with laser microbeam (knife) coupled together can achieve laser-induced cell fusion, the most advanced GM technology is the use of optical tweezers and a scalpel DNA into cells and achieve gene transfer This method can save a lot of resources, shorten time genetically improve the success rate. 1.5 directly applied to the study of animal cells in vivo real-time observation, control and measurement, experimental evidence implement non-contact laser tweezers surgery can penetrate directly into the animal cells in vivo real-time observation, control and measurement, implementation of non-contact operation Experimental evidence, thus opening up the optical tweezers technology research new areas of live animals, provides a new technical means for research and clinical diagnosis in vivo in a living animal in vivo studies of cell growth, migration, and protein-protein interactions between cells and other biological the process of science, medical research and clinical diagnosis of life is of great significance, and therefore in vivo studies technology has been one of the hot vivo studies. 2. Single-molecule research in the field of application of quantitative measurement of biological macromolecules 2.1 Mechanical properties of biological macromolecules are usually tied to the diameter of about 1um polystyrene beads, while medium pellets through the optical tweezers are trapped in an optical trap in. By optical tweezers single molecule twisting, bending, stretching operation to study the mechanical properties. These studies Protein self-acting assembly and between cells is important. 2.2 pairs of biological macromolecules optical tweezers unravel the fine manipulation of DNA molecules wound of biological macromolecules folded conformation depth research; double optical tweezers method of DNA molecules are twisted, knotted, intracellular protein fiber study the molecular mechanics of interaction opens up new ways to track and describe optical tweezers manipulation of nanoscale accuracy and precision observation of specific recognition and binding of optical tweezers love life regulation 2.3 molecular level between individual molecules on has, so Optical tweezers can be the object to be observed as necessary pairing and pairing new changes observed; this made it possible at the nanoscale real-time dynamic studies of single cells that specifically recognize and display its specific molecular mechanisms of interaction, so as to unlock cell-specific molecular recognition provide microscopic information. One study of molecular motors 2.4 Application of optical tweezers in the study of biological macromolecules of the most important results is the study of the power of the original protein. Scientists observed using optical tweezers yuan motion the process of life, and found molecular motor movement is stepping way, and measured step, given as a function of power and speed and ATP concentration of single kinesin molecule produced. NanoTweezer microscope nm laser tweezers manipulation conversion device element 1) Bench NanoTweezer laser control device (NanoTweezerTM Instrument) Desktop NanoTweezer instrument with all optical and microfluidic infrastructure required for operating Optofluidics NanoTweezer system, as well as connections from the chip to the instrument nanotweezer Flow Control Manager plug and play fiber optic and operations. 2) NanoTweezer chip (NanoTweezer Chips): 2.1 contains optical components and micro streamer waveguide core control equipment 2.3 2.2 photonic resonator high optical quality of the optical waveguide chip microfluidic chip contains the NanoTweezer photon resonance device and high optical quality micro flow control the optical waveguide chip. Taking into account the customer expensive samples. Channel volume less than 300 nL (adjustable), hard fiber coupled to the chip is no longer any need for optical alignment, can remain stable throughout the experiment. 3) NanoTweezerTM chip and microscope adapter (Custom Microscope Mounts) specially designed custom microscope adapter so NanoTweezer chip microscope with existing equipment Plug the direct connection. Fluid samples through microscopic flow cell chip go into the lower side of the entrance. The mounting member is less than five minutes, connecting, very convenient to unload. 4) NanoTweezer computer control system NanoTweezer computer control system can use a custom graphical user interface directly manipulate. The graphical user interface is easy to use, can simultaneously control flow and continuous speed and laser power. The interface also provides direct feedback detector power. System parameters 1, the system connectivity: 1. Can the microscope combined with 2. Can laser micro-Raman spectroscopy in combination with 2 capture and manipulation capabilities and research level Upright: new nano-optical tweezers system NanoTweezer, using the world's most advanced integrated optical waveguides and resonant body, laser emitted by the micro-chip to capture and manipulate nano-clump micron particles. You can achieve a variety of applications, such as the operation is much less than conventional optical tweezers sample and keep particle structure is not destroyed; implementation of new types of experiments and analysis of particle capture manipulation size range 2.1: 10nm-5 covers the operation target 2.2 microns. single cells, single molecules, viruses, nucleic acids, nanoparticles, nanotubes and protein 3, the laser: 3.1 laser wavelength (Wavelength): 1065nm 3.2 laser power (Optical Power): 0-500 mW continuously adjustable 3.3 optical interface: FC / APC SMPM 3.4 optically isolated (Optical Isolation): 33-38 dB 4, microscope flow cell: 4.1 Maximum Pressure: 20psi (1psi = 6.895kPa) 4.2 Flow rate: 80nl / min (min) -1000ml / hr (max) 5 , and Raman microscope combined with Annex 5.1 optical tweezers Raman spectroscopy associated link adapter 5.2 OD6 band-stop filter (1064nm) 6, Instrument Size: 8 "(W) X14" (long) x9 "(high) Typical applications: laser tweezers in a single cell, single molecule science research in biomedical optical tweezers use optical tweezers technology to achieve new progress in research animals