Supply clenbuterol rapid test strip

Description: test strip instructions for use: Note 1, the test strips require drying to save, please do not hesitate to ensure that the cap has reclosable. 2, the test environment temperature> 15 ℃ can be. 3, try not to touch the test strip piece central in the white membrane surface. 4, the reaction bottom of the hole or uneven massive crystals near normal. 5, the test strip use, need to first complete part of warmed at room temperature for 30 minutes in order to ensure the correctness of the response. 6, the detection of a sample using a reaction hole, a test piece and a dropper. Unused wells can still be used every times. 7-fold dilution buffer preparation: 10 times with distilled water, concentrated dilution buffer dilution ratio of 1:9 (ie 1mL concentrate + 9 mL of distilled water), you can as a sample diluent. B, sample preparation: 1. Serum: extract the blood of animals to be seized, centrifugation or standing whichever is more transparent in the supernatant (serum layer) (if the serum excessive hemolysis, please serum doubled and then diluted in distilled water experiment, otherwise it will lead to reagent red piece is too deep, seriously affect the interpretation of results). (2) urine: direct urine test, if turbidity Please centrifugal supernatant. (Eg, better) with distilled water 1:1 dilution effect. (3) meat (10 times diluted): Take 1g has homogeneous sample placed in a centrifuge tube, and then by adding 0.01 N of HCl was 1.5mL, vortex 1 minute. For 1 hour, centrifuged for 20 minutes (3000rpm), the supernatant dilution buffer 1:3 (four times) dilution for analysis. (Dilution factor can be adjusted as needed) 4. Heart, liver and other organs (10-fold dilution, boiling): take 1g homogeneous sample placed in the centrifuge tube, and then by adding 0.01 N of HCl was 1.5mL, vortex 1 minute. For 1 hour, heated to 80 ℃ water bath for about 3 minutes, and centrifuged for 20 minutes (3000rpm). Supernatant and dilution buffer 1:3 (four times) and diluted for analysis. (Dilution factor can be adjusted as needed) 5. Feed: take 1g ground test feed by adding 10mL diluent, severe shock, shake. 3000rpm centrifuged for 10 minutes, the supernatant 100uL, 10-fold dilution with dilution buffer. Take 100uL for analysis. (Dilution factor can be adjusted as needed) C steps: (sample more than one, please do the corresponding samples and wells number) 1, a drop of the sample: a dropper (or pipette) absorption samples, using a hanging drop (only a drop, not more; or directly into the pipette 40mL) in the corresponding reaction of the hole bottom (do not touches the pore wall), then the reaction hole quickly and effectively in the plane shaking earthquake of at least 5 laps to the scour hole wall overlying the drug layer. 2, back to the dissolved one minute: to stand for about one minute after the shaking, the hole pharmaceutical completely dissolved, and then quickly and forcefully shaking earthquake of at least five laps to make the mixed samples dissolved Pharmacy. 3, reaction 10 minutes: roll over and then put it aside for at least about 10 minutes, so that the sample with the pharmacy adequate response. 4, Paopian 8 to 10 minutes: Take a test piece at the end of the white insert end inserted into the wells. 5, interpretation: interpretation after 8-10 minutes. Interpretation: (due to the samples after pre-dilution based on the sample concentration must again multiplied by the dilution factor) case 1-T line than the C line deep or as deep as to indicate a low concentration of clenbuterol in samples The in 2ppb. The case 2-T line is lighter than the C line, indicating that the concentration of clenbuterol in samples of higher than 2ppb. Case 3 - If the C line completely did not appear, then the test fails. Note: The test strips can also be used to test samples of salbutamol (Salbutamol) content, methods of operation with the detection of clenbuterol, but in a slightly different interpretation of the results. : Scenario 1-T line than the C line deep or as deep as that of salbutamol in the sample concentrations below 1ppb. Situation 2-T line is lighter than the C line, said samples salbutamol concentration higher than 1ppb. Case 3 - If the C line completely did not appear, then the test fails